Abstract for Invited Talk by Randall T. Moon



STRATEGIES FOR IDENTIFYING AND VALIDATING NEW COMPONENTS OF SIGNAL TRANSDUCTION NETWORKS


Randall T. Moon, HHMI, University of Washington


Wnts are secreted glycoproteins that act as ligands to activate receptor-mediated signal transduction pathways. These pathways are involved in embryonic development, tissue homeostasis and regeneration in adults, and in a variety of diseases. While originally defined on the basis of a somewhat linear genetic pathway in Drosophila, more recent approaches have been changing our understanding of this signaling network, and changing how we screen for additional modulators of Wnt signaling. I will first demonstrate that both siRNA screens and proteomic screens have yielded valuable insights into Wnt signaling. However, I will then make the argument that integrating the hits from proteomic, siRNA, and small molecule screens is a much more powerful screening approach.