STRATEGIES FOR IDENTIFYING AND VALIDATING NEW COMPONENTS OF SIGNAL TRANSDUCTION NETWORKS
Randall T. Moon, HHMI, University of Washington
Wnts are secreted glycoproteins that act as ligands to activate receptor-mediated signal transduction pathways. These pathways are involved in embryonic development, tissue homeostasis and regeneration in adults, and in a variety of diseases. While originally defined on the basis of a somewhat linear genetic pathway in Drosophila, more recent approaches have been changing our understanding of this signaling network, and changing how we screen for additional modulators of Wnt signaling. I will first demonstrate that both siRNA screens and proteomic screens have yielded valuable insights into Wnt signaling. However, I will then make the argument that integrating the hits from proteomic, siRNA, and small molecule screens is a much more powerful screening approach.